Showing posts with label embryo transfer. Show all posts
Showing posts with label embryo transfer. Show all posts

April 23, 2014

New Zealand agricultural history. No 7. Importing exotic sheep breeds

Quarantine on Somes Island
Sheep moved from Somes Island to Mana Island
Sheep moved from Mana Island to Crater Block

By Dr Clive Dalton
  
From Somes Island to Mana Island

Somes Island 2014.  Former buildings used to house livestock.  
Photo Jim Hammonds
 Tim Harvey remembers that when the Somes Island quarantine flock got to around 500 sheep, the proposed move to Mana became urgent, even though the ink wasn’t dry on the lease agreement, and staff moving there had to live in old sheds in awful conditions till they got accommodation organised.    

John Dobbie also remembers the  work involved getting this underway with some massive movement of shingle off the beach to form a 2m deep base for the woolshed and yards.  He had the job of sorting out the contract for suitable tractors to do the work, with prime emphasis on safety and the power required. Choice at the time was restricted between Russian and Italian models and the Russians lost!

About 70 of the purebreds from the 104 that arrived on Somes eventually got to Mana, the balance including all the East Friesians ending their days on Somes.

Work to prepare Mana 

Mana Island - view from mainland at Mana. 
 Photo Jim Hammonds
 It was obvious that there would be a massive amount of work ahead on Mana, needing enormous attention to detail. Tim Harvey from Somes was already on the job at Mana and Dr Alan Carter Head of the Ruakura Genetics Section headhunted John Dobbie to help out. 


 
John had been a MAF Sheep and Wool officer in the Farm Advisory Division and like Tim, they were legends for their attention to detail in the sheep and wool world. 

With all the work that had gone into the exercise this far, any stuffups over collecting data would have been the final straw!  The job required MAF’s ‘top guns’ and the Harvey-Dobbie team were the men for the job.




The first challenge on Mana was to produce purebreds from matings of the purebred ewes and rams that went from Somes to Mana.  These were superovulated and the resulting embryos put into Romney and Coopworth ewes sent to Mana as recipients.

Dr Robin Tervit very proud of the first  purebred Finn (left) and Texel (middle) and Oxford Down lambs (right) from the  embryo transfers done on Somes Island.  
Photo: Robin Tervit
 And then the Romneys and Coopworth ewes were mated to the purebred rams to produce F1 crossbreds, and these F1’s in future years were subsequently used to breed F2s and F3’s for mass supply to the industry which took over 3 years.  After all, that was the prime aim of the whole project - to get these genes to farmers in a hurry.

So the procurement of the Romney and Coopworth ewes was another very important job that Tim Harvey and John Dobbie had to arrange. Tim Harvey remembers the Romney ewes coming from MAF research stations at Woodlands, Tokanui and Whatawhata as well as from Lands and Survey flocks at Waihora near Taupo.  Tim and John can’t remember where the Coopworths came from. 

Embryo transfer on Mana
MAF reproductive physiologists Drs Robin Tervit, John Smith from Ruakura and Dr Jock Allison from Invermay did all the ET work on Mana, and Tim remembers some good social evenings after a day of looking down laparoscopes.

Visitors to Mana
 
The wharf at Mana - departure point for Mana Island. 
Photo Jim Hammonds


 But working on Mana was not only dealing with sheep. This was probably the easiest part. The tricky bit was dealing with the droves of bureaucrats, politicians, scientists, vets, official visitors and plain ‘rubber neckers’ who were involved in the exercise, or just wanted to see what was going on.
I once tried to nosey a trip to Mana wearing my MAFQual ‘information coordinator’ hat, but it never seemed to happen. The advice was always to pick a fine day and not one when the wind was howling direct from the Antarctic ice smelling of penguins. 

Flock expansion on Mana
Tim Harvey remembers the flock (purebreds and crosses) on Mana eventually getting to around 1900 animals. John Dobbie well remembers the strict instructions from Sam Jamieson that no sheep should ever be allowed to die on Mana unless it had been given a final blessing by a veterinary officer, and after death it’s brain removed and sent in formalin to Wallaceville Animal Health Lab in Lower Hutt for examination. Sam  was clearly aware of the need to be alert and act fast if any nasties showed up, especially the dreaded Scrapie.

'There's a moose loose in the hoose' 
 
As well as being out on the limited pasture the Mana flock had to be provided with a lot of concentrate feed, so farm staff had another population to deal with – mice!  John Dobbie said that the mice were so bad that they’d dug burrows all over the place and there was danger of ground collapsing when you walked over it. 


John says this actually happened when the Tim Harvey's house was so undermined with mice runs, that it slipped off it's blocks and slid into the single men's quarters.
John Dobbie said that a trick they used was to cut the lid off a 20-gallon petrol can and arrange a carefully balanced plank for the mice to walk along to get at some sheep nuts in the bottom.  Their combined weight pushing to get the feed collapsed the plank, and the can would be nearly filled with mice overnight.   

Somes Island in its maximum quarantine days. Manager's house on extreme left and woolshed and yards on right of picture. 
 Photo by Ken Seecombe
Dr Robin Tervit remembers the mice.  He says that when he, Dr John Smith from Ruakura and Dr Jock Alison from Invermay Research Centre, together with Tim Harvey and the genetics staff were conducting embryo transfers on Mana in about 1975 the Island was alive with mice.  

Robin says that they  had a daily battle to rid their accommodation of the pests. 'Att night they came back in and skittered across our faces as we tried to sleep! When we went out to check the donor mating at night, as soon as we turned the lights on in the barns, the whole floor took off as the mice rushed away'. 

'Tim and his crew poisoned the animals and I recall them filling at least one 44 gallon drum with mice from a nights kill. And then there were the penguins that constantly called for mates from under our accommodation – not conducive to sleeping' . The native Mana Island Weta were also at risk from these little free loaders.

Mana to Crater Lands and Survey block


Eric Gibson
As the Mana flock increased, stock were then overflowed on to the Crater Lands and Survey block near Rotorua under Superintendent Eric Gibson.  Eric had the responsibility to double fence the property to stop anything getting in or out, while quarantine control and all associated ‘goings-on’ were still under MAF with some MAF Livestock Officers resident at Crater.    
                                              
Eric was a no-nonsense bloke and he was keen on new developments, but like all regional superintendents, he didn't like his territory interfered with.  He had a special warm relationship with the Minister of Lands, Duncan McIntyre. They both liked a wee dram and shooting quail.


Things were going nicely on Mana as the breeding programme continued.  Things were going well at Crater too, where the sheep from the continuing crossbreeding programme were being multiplied in numbers.  The Ruakura Genetics’ boffins were getting some good data for early reports to farmers and papers published in recognised journals, and everyone was keen to hear what was going on.  The farming media was also enjoying covering all this new information.

Sick animal protocol
 Any sick animals at Crater had to be post-mortemed with special emphasis on examining brain tissue for signs of Scrapie.  Up until September 1976, 533 brains were examined at the Animal Health Reference Laboratory at Wallaceville with negative results.  MAF Head Office veterinarian Dr Peter O’Hara told me that the Wallaceville staff got sick of the sight of sheep’s brains!

Wallaceville which made a massive contribution to animal science in New Zealand.  Under 'restructuring' in 2013 it was closed and put up for sale or lease by AgResearch. 
Photo by Allen Heath



New Zealand agricultural history. No 13. Importing exotic sheep breeds


 Harvesting the exotic sheep embryos in Denmark and Finland
Dr Robin Tervit’s involvement
More of Robin Tervit's memories 

 By Dr Clive Dalton

 After the disaster of MAF's first importation of exotic sheep breeds as live animals in 1972 , the second importation in 1984 was only be allowed by MAF and the Maximum Security Quarantine Advisory Committee (MSQAC) as frozen embryos and frozen semen.  Denmark and Finland had the sheep the New Zealand MAF Research Geneticists wanted, and their health standards were also acceptable for importation.

 Harvesting embryos

Harvesting the embryos involved massive cooperation and help from people in both countries to help Dr Robin Tervit from Ruakura who had overall responsibility to harvest the frozen embryos for the importation.

 Photo shows Dr Robin Tervit recovering embryos from the exposed uterus and fallopian tubes of a donor ewe in Finland. Dr Vesa Rainio from Finland is assisting.  Stuart McDiamid in background.  Photo: Robin Tervit.


Robin remembers being called to Agricultural Research Director Dr John Hutton’s office to discuss the ET work he was about to undertake in Europe, and coming away with the clear message that his rear end would be in trouble if he didn’t get good embryo survival results post freezing. Hutton was clearly under the gun from higher up the MAF hierarchy, and he was never a man who liked to back down on issues.  Fortunately, Robin got good results with help from local colleagues.

In the  programme, the embryos for export had to be washed 10 times before freezing to ensure they were not contaminated by any disease organisms not wanted in New Zealand.  This was a fairly new development at the time.  Semen from each breed was also processed and frozen for transport to New Zealand.

Dr Robin Tervit’s involvement
I asked Robin some questions about what he’d been up to with his ET work in Denmark and Finland.These are his replies:

How long were you working on the sheep?
Late August 1984 through late October in Denmark, and late September through late November in Finland. The Danish ewes were divided into 3 groups and one group treated per week for 3 weeks from late August. The interval from start of treatment to surgery was about 40 days and so the ewes were operated on and embryos recovered during the first 3 weeks in October. 

We left Denmark during the early treatment phase of the Danish ewes, and started the Finnish ewe treatment about a month later than the Danish ewes (i.e. in late September/early October). Finn surgery was about a month later than in Denmark (November). So, I was away from NZ for just over 3 months.

Were there any Kiwis helping you? 
Yes, MAF veterinarian Stuart MacDiarmid was with me for the whole time. He was responsible for animal health issues and also did a lot of the surgery on the donors. 

I recovered the embryos from the uterine washings and then processed them through the freezing process. I accompanied the frozen embryos back to NZ.  



 Full technical details are given in the 1986 paper below.
Tervit H R, Baker R L, Hoff-Jorgenson R, Lintukongos S, MacDiarmid S C, Rainio V. 1986. Viability of frozen sheep embryos and semen imported from Europe.  Proceedings of the New Zealand Society of Animal Production 46:245-250.

More of Robin Tervit's memories  
 Denmark
 The donors were housed in a lovely historic barn which was not being used by anyone. I can still see the Danes' look of disbelief when we took to the pens with a spanner and sledge hammer to make them large enough to accommodate the large donors and their rams. 

Surgery was conducted at the vet school in Copenhagen and went very well. The only problem was that the vet students assisting us started to get difficult to commit to assisting with the surgery. We discovered that they were not getting paid – a talk to Leyden Baker soon sorted this out.

Stuart McDiarmid and I stayed in a hotel in Copenhagen and , after traveling to Finland to begin the donor hormone treatments, we returned to our hotel to find that we had been evicted and replaced with some refugees from the Middle East. No attempt had been made to find us alternative accommodation, so we had to go and find somewhere else to live.
Finland

Conditions were nowhere near as salubrious as in Denmark – but everything worked well showing that you don’t need fancy facilities to achieve good results. The animal housing barn was old and the water pipes froze, with the result that we had to carry drinking water to the animals when it got cold. The donors were carried to and from the surgery in a VW Combi van. The surgery was in an adjacent building and the embryologist (me) was squashed into one corner.

Stuart and I were accommodated in a house near the animal barns. It had a sauna but since we did not share the Finnish enthusiasm for hot rooms we used it mainly to dry our clothes. We did have some saunas with Finnish people, and I still remember the heat hitting the lining of the nose – boy they liked the temperature high.

It snowed while we were in Finland and though it was cold this was balanced by the brilliant northern lights.

 Roger Marshall's role.
Noted Coopworth breeder and New Zealand Meat Board member as asked to visit Denmark and Finland in 1984 on behalf of the NZ United Breed Societies to approve the sheep that were going to provide the genetics (embryos and semen) for the  NZ sheep industry.  It was an important responsibility and Roger was certainly an ideal person to do the job. 

He worked in cooperation with breed society executives in both countries and Dr Leyden Baker from MAF.  He reported his work to the UBS on 11/9/1984.